Expression of NKp30, NKp46 and CD85j is involved in poor functionality of NK cells from Colorectal Cancer Patients

ROCCA, YAMILA SOL; JULIÁ, ESTEFANÍA PAULA; PAMPENA, MARÍA BETINA; BRUNO, LUISINA; HUERTAS, EDUARDO; SÁNCHEZ LORIA, FERNANDO; PAIROLA, ALEJANDRO; RIVERO, SERGIO; MORDOH J; ESTRELLA M LEVY

Congreso; IV LASID Meeting LXIII Argentinean Immunology Society Meeting II French-Argentinean Immunology Meeting; 2015

BackgroundNatural Killer (NK) cells are part of the innate response and their activity is regulated through surface receptors triggering activating or inhibitory signals. Particularly, NK cells have the ability to lyse tumor cells. Previously, we described that blood NK cells from colorectal cancer patients (CRC-NK) present an altered phenotype. The aim of this study is to evaluate the impact of this abnormal phenotype in their lytic activity. MethodsCRC-NK cells were compared with healthy donors NK cells (HD-NK). NK cell phenotype was determined by flow cytometry. NK cell mediated lysis against CRC cell line DLD-1 was performed using calcein-labeled assay. In addition, receptor blockade assay was evaluated using label-free xCELLigence system. Statistical analysis was performed using the Mann-Whitney t test. ResultsExpression levels of activating natural cytotoxicity receptors NKp30 and NKp46, and of the inhibitory CD85j receptor in CRC-NK are associated (p