Altered phenotype and function of NK cells in breast cancer patients: CD85j overexpression as a mechanism of immunosuppression.

ROBERTI, MARÍA PAULA; ROCCA, YAMILA SOL; PAMPENA, MARÍA BETINA; ARRIAGA, JUAN MARTÍN; PODHORZER, ARIEL ; BARRIO, MARÍA MARCELA; FAINBOIM, LEONARDO; MORDOH, JOSÉ; LEVY, ESTRELLA MARIEL

Congreso; LXI REUNION ANUAL DE LA SOC ARGENTINA DE INMUNOLOGIA; 2013

In the present study we aim to associate phenotypic deregulation found in BC patients with abnormal NK cells activities. We used our previously established model of Natural Killer (NK) cells an based immunotherapy, focusing on the immune activity triggered by the monoclonal antibody (Ab) Cetuximab against triple negative breast cancer (TNBC) cells. We compared the expression of 13 activating/inhibitory receptors of BC peripheral blood NK cells with those from normal female donors (ND) by flow cytometry. For functional experiments, PBMC were isolated and assayed against different tumor targets including TNBC cells coated with Cetuximab for Ab-dependent cellular cytotoxicity (ADCC) assessment. Advanced BC patients? peripheral blood NK cells display diminished levels of ADCC compared to those from ND (p