Effects of parenteral supplementation of antioxidant minerals and vitamins in liver nf-κb activity and insulin-signaling of dairy cattle during the transition period.

ANGELI, E.; SCHNEIDER, A; MATILLER V.; REY, FLORENCIA; ORTEGA H.H.; HEIN G.

Mar del Plata

Otro; . LXVIII Reunión Anual de SAIC; 2023

Sociedad Argentina de Investigación Clínica

The transition period (last 3 weeks of prepartum until 3 weekspostpartum) is the most critical stage in the lactation of dairy cows,characterized by a proinflammatory state, oxidative stress and insulinresistance. The aim of this study was to evaluate the effectsof parenteral vitamin and mineral supplementation on the hepaticinsulin signaling pathway, a relevant proinflammatory factor in theliver, and systemic antioxidant enzymes in cows during the transitionperiod. The supplemented group (SG; n = 11) received injections of5 ml of the vitamin supplement ADAPTADOR® Vit and 5 ml of themineral supplement ADAPTADOR® Min (Biogenesis Bagó, Bs. As.;vitamin A palmitate 3.5% and vitamin E acetate 5%, copper edetate1%, zinc edetate 4%, manganese edetate 1% and sodium selenite0.5%) on -60, -30 and 7 days relative to calving. The control group(CG; n = 11) received two injections of 5 ml of 0.9 % sodium chloridesolution. Whole blood with heparin was used to measure superoxidedismutase (SOD) and glutathione peroxidase (GSH-Px) activities bycommercial kits, following the manufacturer’s instructions (RANSODand RANSEL, Randox Laboratories Ltd., Crumlin, UK). The liverprotein expression of insulin receptor (IR), insulin receptor substrate1 (IRS-1), protein kinase B (also known as Akt), nuclear factor-κB(NF-κB), and their phosphorylated forms were analyzed by Westernblot. Results showed that the GSH-Px activity was higher in theSG than in the CG (P < 0.05). No differences between groups wereobserved in SOD activity (P > 0.05). The activation of NF-κB washigher in SG than in CG (P < 0.05). No differences between groupswere observed in the insulin signaling components (P > 0.05). Theseresults suggest that the vitamin and mineral supplementation providedto dairy cows had beneficial effects on the antioxidant systemand liver inflammatory state during the transition period.