Isolation and characterization of an abietane diterpene from Coleus neochilus with potent antiproliferative activity on a human breast cancer cell line.

CARLA LUCIANA MAYORA JUSTEL; TAMARA VALLADARES; CAVALLARO, VALERIA; ANA PAULA MURRAY; ISABEL ALICIA LÜTHY; MARÍA DEL CARMEN ESANDI; ARIANA BRUZZONE; NATALIA P. ALZA

Mar del Plata

Congreso; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental.; 2023

Sociedad Argentina de Investigación Clínica; Sociedad Argentina de Biología; Asociación Argentina de Farmacología Experimental; Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio;

Coleus spp. have diverse ethnobotanical applications, with their most prevalent use being attributed to their medicinal properties. We have previously described the antiproliferative effect of the ethanolic extract from C. neochilus (also known as “boldo rastrero”) on differ- ent human breast cancer cell lines. The present study is focused on the extraction and isolation of compounds responsible for this effect. The ethanol extract (BRET) was subjected to solvent-solvent ex- traction, yielding four different sub-extracts: hexane (BRHX), chlo- roform (BRCL), ethyl acetate (BRAE), and methanol-water (BRAM). Remarkably, incubation with BRAE (50 μg/ml) was more effective in reducing the viability of human breast cancer cell line MCF-7 than the other fractions (BRET: 23%, BRHX: 29%, BRCL: 52%, BRAE: 68%, and BRAM: 10% reduction; p