The presence of plasmid pYV impacts on the immunometabolic response of Yersinia enterocolitica infection

MARISOL VELAZQUEZ; JUAN EDUARDO SILVA; MARÍA SILVIA DI GENARO

San Luis

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología; 2023

Sociedad Argentina de Inmunología

Yersinia enterocolitica (Ye) is a zoonotic pathogen that causes bacterialgastrointestinal infections in humans. The virulence plasmid, termed pYV(plasmid ofYersinia virulence) encodes a type III secretion system essential forthe delivery of anti-host virulence factors collectively known as Yops (Yersiniaouter proteins). Although the majority of infections are self-limiting,immunocompromised hosts have higher risk of developing significant disease.Immunometabolism is a key mechanismto develop both adaptive and innateimmune responses by triggering functional and metabolic reprogramming inimmune cells. However, the role of pYV in metabolic reprogramming remainsunclear. Here, we examined the relationship between the presence of pYV withthe immune and metabolic responses during the course of Ye infection in vivounder normal and TNFR1-deficiency conditions. C57BL/6 wild-type (WT) orTNFR1-deficient (KO) mice were orally infected with Ye serotype O:8 plasmidbearing (pYV+), or plasmid cured (PC) WAP strain by gavage. Body weight, foodintake, glycemia, cholesterol and serum TNF levels were evaluated during thecourseof the infection. Cell recruitment was analyzed by flow cytometry. Ye O:8pYV+ infected WT and KO mice exhibited significant weight loss (p