Inositol hexakisphosphate (ip6) production is mediated by YopP and n-glycans of macrophages participate in the nitric oxide regulation during Yersinia enterocolitica infection

JUAN AGUSTÍN GARAY; JUAN EDUARDO SILVA; BRENDA JOFRE; MARÍA SILVIA DI GENARO ; ROBERTO CARLOS DAVICINO

San Luis

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología; 2023

Sociedad Argentina de Inmunología

Yersinia enterocolitica (Ye) is a pathogenic bacterium that cause intestinalinfections. Different Yops (Yersinia outer proteins) are main virulent factors of Ye.YopP is activated by the host factor inositol hexakisphosphate (IP6) and regulatesnitric oxide (NO) production during Ye infection. Recently, it has been found thatthe glycosylation of murine macrophage surface receptors plays a pivotal role inthe interaction with pathogens. Here, we investigated the IP6 production and theparticipation of glycans on NO regulation by YopP during Ye infection. To explorewhether YopP is activated by IP6 binding, we measured IP6 at different timespost infection (15, 30, 45, 60 min) in lysates of murine peritoneal macrophages(MΦ) infected with Ye wild-type (Ye wt) or with YopP-deficient (Ye ΔyopP). Weobserved that the infection with Ye wt stimulates IP6 production at 30 min postinfection (p