PERINATAL PROGRAMMING WITH HYPERTONIC SODIUM: EFFECTS ON SODIUM INTAKE AND HYPERTENSION INDUCED BY DOCA-SAL MODEL

PORCARI, CINTIA Y.; CAEIRO, XIMENA E.; GODINO ANDREA

Congreso; SAFIS: reunion de la sociedad argentina de fisiologia; 2023

Introduction: Our previous results indicate that voluntary maternal intake of hypertonic NaCl during gestation/lactation affects the offspring sodium/water intake, brain mRNA expression of angiotensin receptor 1a(Agtr1a), vasopressin,basal number of renal glomeruli, renal gene expression of the TRPV1 channel and Agtr1a and causes a sustained increase of blood pressure after a sodium overload. Objective: Our aim was evaluating the effects of perinatal imprinting induced by voluntary consumption of hypertonic NaCl on: -the pattern of hypertension onset in adult offspring, using the desoxycorticosterone acetate and Salt (DOCA-Salt) hypertensive model; - the 1% NaCl intake; - the body size and brain mRNA expression of Agtr1a, and serotonin receptor 2c(Htr2c) in the sufornical organ and paraventricular nuclei.Methods: The perinatal manipulation period embraced dams from 1 week before conception until offspring at postnatal day 28 when the animals (Wistar rats) receive access to NaCl (0.45M), food, and water (MP-NaCl group) or food and water (Control: MP-Ctrol). On 50 postnatal day, male offspring were subjected to DOCA treatment for 4 weeks (25 mg/kg twice a week) and water was replaced with a 1% NaCl solution. The systolic blood pressure was evaluated in conscious rats using the non-invasive tail cuff method, intake volume of 1% NaCl, and brain Agtr1a and Ht2c receptors mRNA expression .Results: the DOCA-salt hypertensive treatment to MP-NaCl and MP-Ctrol animals did not differentially modify blood pressure between them and it did not affect the brain expression of Agtr1a and Ht2c receptors, but salt consumption significantly increased in PM-NaCl groups [F(1,10)= 11.907;p=0.006].Conclusion: These results, in addition to previous data, indicate that the availability of a rich source of NaCl solution during the perinatal period induces long-term changes in osmoregulatory mechanisms and circuits, altering the functioning of the renal and brain angiotensin and vasopressin systems, which together modulate behavioral, endocrine, and renal responses to achieve hydroelectrolytic and cardiovascular homeostasis after different challenges (including DOCA-SAL).Acknowledgements: CONICET, FONCyT and SECyT FISIOLOGÍA CARDIOVASCULAR-HIPERTENSIÓN