Diferential effect of chronic stress exposure on diabetes incidence on male and female NOD/ShiLtJ mice. Participation of gut microbiota and intestinal permeability

WYDRA L; GONZALEZ MURANO MR; BIANCHI MS; WALD MR; GENARO AM; RUBINSTEIN MR

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Anual de la Sociedad Argentina de Fisiología; 2023

Sociedad Argentina de Fisiología (SAFIS)

Introduction: Type 1 diabetes (T1D) is an autoimmune disease characterized by impaired insulinsecretion. It has been recognized the contribution of psychosocial factors (including chronic stressexposure) in T1D in its development. Gut microbiota is the group of microorganisms (commensal,symbiotic and pathogenic) that we find in our gut. It participates in multiple functions and anassociation between unbalanced microbiota and several diseases, including diabetes, has beenreported. NOD/ShiLtJ mice are a model for autoimmune T1D, where the animals spontaneouslybecame diabetic. There is a marked sexual dimorphism: a higher T1D diabetes incidence infemales than in males. Objectives: the aim of the present work was to study the effect of chronicstress in diabetes development and to characterize the microbiota alterations in NOD/ShiLtJ micein females and males. Methods: Two-month old NOD/ShiLtJ mice were subject to chronic stress(CS) by the application of aleatory and unpredictable stressors. Results: females NOD/ShiLtJmice exposed to CS showed an increase in diabetes incidence (p