The flavonoid 2’nitroflavone reduces viability and migration in human endothelial cells

BENSO MIRIAM; VACHETTA, VANINA S.; SARRIAS, LUCIANA; MARDER, MARIEL; TRONCOSO, MARÍA F.; ELOLA, MARÍA TERESA

CIUDAD AUTONOMAA DE BUENOS AIRES

Congreso; REUNION 2023 DE LA SOCIEDAD ARGENTINA DE FISIOLOGIA; 2023

Argentinean Physiological Society and the Latin American Association of Physiological Sciences

THE FLAVONOID 2’-NITROFLAVONE REDUCES VIABILITY AND MIGRATION IN HUMAN ENDOTHELIAL CELLS.Benso M1, Vachetta VS1, Sarrias L1, Marder M1, Troncoso MF1, Elola MT1.1. Instituto de Química y Fisicoquímica Biológicas (IQUIFIB, UBA-CONICET), Facultad de Farmacia y Bioquímica, UBA.Introduction: Flavonoids are polyphenolic compounds that cause several antineoplastic effects. We previously described that the synthetic flavonoid 2’-nitroflavone (2’NF) inhibits proliferation and migration of human triple-negative breast cancer (TNBC) cells. Objectives: Our aim pointed to find out 2’NF effects on endothelial cells. Methods: We employed EA.hy926 human endothelial cells to perform viability (MTT) and migration (wound-healing) assays. Two experimental approaches were conducted: A) direct treatments with 2’NF (1.25-40 µM) or vehicle on endothelial cells, and B) endothelial cells treated with conditioned media (CM) derived from TNBC cells pre-incubated with 2’NF (1-25 µM) or vehicle. Results: EA.hy926 cell viability was significantly reduced with 2.5 µM 2’NF (77.98±4.06%; n=5; p0.05) were selected. In scratch assays, in the presence of 1% FBS, direct treatment (A approach) with 1.25 µM 2´NF significantly reduced cell migration (vehicle: 100%; 2’NF: 80.78±3.39%; n=4; p