Bacterial Populations Of A Nitrifying Activated Sludge

EVA L. M. FIGUEROLA; LEONARDO ERIJMAN

Gold Coast, Queensland, Australia

Conferencia; 4th IWA Activated Sludge Population Dynamics Specialist Conference; 2005

International Water Asociation

Abstract The composition of the microbial community present in activated sludge of an industrial treatment plant receiving pretreated wastewater of an oil refinery was investigated by the full-cycle rRNA approach. A total of 139 16S rRNA gene clones were sequenced for phylogenetic analysis. The microbial community composition of the activated sludge was also determined by using rRNA-targeted oligonucleotide probes for fluorescence in situ hybridization. In both cases, Alphaproteobacteria represented the most abundant population, followed by Betaproteobacteria and Acidobacteria. Actinobacteria, which were also detected population, followed by Betaproteobacteria and Acidobacteria. Actinobacteria, which were also detected in situ hybridization. In both cases, Alphaproteobacteria represented the most abundant population, followed by Betaproteobacteria and Acidobacteria. Actinobacteria, which were also detectedBetaproteobacteria and Acidobacteria. Actinobacteria, which were also detected in situ using a group-specific probe, were represented albeit in low number in the clone library.using a group-specific probe, were represented albeit in low number in the clone library. Verrucomicrobia, Bacteroidetes and Firmicutes where each represented with a single clone. FISH analysis demonstrated the presence of Deltaproteobacteria and Planctomycetales, for which representative clones were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. demonstrated the presence of Deltaproteobacteria and Planctomycetales, for which representative clones were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. where each represented with a single clone. FISH analysis demonstrated the presence of Deltaproteobacteria and Planctomycetales, for which representative clones were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Deltaproteobacteria and Planctomycetales, for which representative clones were not found in the 16S rRNA gene clone library. Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. . Although full nitrification was observed, Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. for the possibility that yet undiscovered bacteria are responsible for the observed nitrification. Nitrosomonas-related sequences, allowing for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.