INVESTIGADORES
FIGUEROLA Eva Lucia Margarita
congresos y reuniones científicas
Título:
Bacterial Populations Of A Nitrifying Activated Sludge
Autor/es:
EVA L. M. FIGUEROLA; LEONARDO ERIJMAN
Lugar:
Gold Coast, Queensland, Australia
Reunión:
Conferencia; 4th IWA Activated Sludge Population Dynamics Specialist Conference; 2005
Institución organizadora:
International Water Asociation
Resumen:
Abstract
The composition of the microbial community present in activated sludge of an industrial treatment plant
receiving pretreated wastewater of an oil refinery was investigated by the full-cycle rRNA approach. A
total of 139 16S rRNA gene clones were sequenced for phylogenetic analysis. The microbial community
composition of the activated sludge was also determined by using rRNA-targeted oligonucleotide probes
for fluorescence in situ hybridization. In both cases, Alphaproteobacteria represented the most abundant
population, followed by Betaproteobacteria and Acidobacteria. Actinobacteria, which were also detected
population, followed by Betaproteobacteria and Acidobacteria. Actinobacteria, which were also detected
in situ hybridization. In both cases, Alphaproteobacteria represented the most abundant
population, followed by Betaproteobacteria and Acidobacteria. Actinobacteria, which were also detectedBetaproteobacteria and Acidobacteria. Actinobacteria, which were also detected
in situ using a group-specific probe, were represented albeit in low number in the clone library.using a group-specific probe, were represented albeit in low number in the clone library.
Verrucomicrobia, Bacteroidetes and Firmicutes where each represented with a single clone. FISH analysis
demonstrated the presence of Deltaproteobacteria and Planctomycetales, for which representative clones
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
demonstrated the presence of Deltaproteobacteria and Planctomycetales, for which representative clones
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
where each represented with a single clone. FISH analysis
demonstrated the presence of Deltaproteobacteria and Planctomycetales, for which representative clones
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Deltaproteobacteria and Planctomycetales, for which representative clones
were not found in the 16S rRNA gene clone library. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
. Although full nitrification was observed,
Betaproteobacterial ammonia-oxidizer were not represented in the clone library. Only PCR-DGGE
analysis using AOB-specific primers revealed the presence of Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.
Nitrosomonas-related sequences, allowing
for the possibility that yet undiscovered bacteria are responsible for the observed nitrification.