Detrimental effects of lindane on the bovine oviductal epithelial cells

DE BOECK, MAXIMILIANO; ANGEL SPIESS, IGNACIO A.; ROLDÁN OLARTE, MARIELA; VELLA, MILDA A.; CUOZZO, SERGIO A.; VALDECANTOS, PABLO A.

Mar del Plata

Congreso; Reunión Conjunta de Sociedades de Biología; 2023

SAIC/ SAB/ AAFE7 AACYTAL

Lindane, the γ-isomer of the hexachlorocyclohexane, was a widely used organochlorine pesticide in agriculture and public health. Due to its high environmental persistence, bioaccumulation, and toxicity, in the present work we investigate its effects on primary cultures of the bovine oviductal epithelial cells (BOEC), focusing on cell viability, proliferation, migration, and genotoxicity. Different lindane concentrations (12.5 μM, 25 μM, 50 μM, 100 μM and 200 μM) were tested on 80% confluent BOEC monolayers in DMEM 5% FSB at 38.5°C, 5% CO2 and 100% humidity. Then cell viability was evaluated by the trypan blue exclusion test. Cell viability was above 86% at all the lindane concentrations assayed. However, concentrations starting from 25 µM exhibited a significant decrease in cell number/mL compared to the control (p