POTENTIAL OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES (SPIONs) AS RADIOSENSITIZER ON CANCER THYROID CELLS

PERONA, M.; GRISSI, C.; NIEVAS, S.; GABET, M.; THOMASZ, L.; OGLIO, R.; RODRIGUEZ, C.; ROSEMBLIT, C.; CREMASCHI, G.; DAGROSA, M.A.; DURÁN, H.; JUVENAL, G.; IBAÑEZ, I.L.

Congreso; Reunión Anual de Sociedades de Biociencias 2023; 2023

Thyroid carcinomas are generally well-behaved malignancies that respond to standard treatments. However, a subset of thyroid carcinomas of follicular epithelial origin (well-differentiated, WD; poorly differentiated, PD; or anaplastic, A) is highly aggressive. External beam radiotherapy could be a treatment option for high risk patients. Superparamagnetic iron oxide nanoparticles (SPIONs) have been used in cancer diagnosis and therapy. SPIONs can increase reactive oxygen species(ROS). This characteristic could be combined with radiotherapy to optimize the clinical outcome. The aim was to study the radiosensitizing properties of SPIONs in thyroid cells. Methods: SPIONs were synthesized and stabilized by methyl-poly(ethylene glycol) (mPEG). Thyroid cancer (WD: TPC-1, PD: WRO and A: 8505c) cells were incubated with different concentrations of mPEG-coated SPIONs. Cell viability was measured by MTT method. Intracellular SPIONs content by measuring the Fe concentration per cell was performed by ICP-AES at 2, 4 and 24 hours. Intracellular ROS levels using the fluorescent dye 2´,7´-dichlorofluorescein-diacetate (DCFH-DA) and survival fraction at 2 Gy (SF2) were measured. Results: 24 hours incubation with SPIONs did not affect cell viability (0-100 µg/ml). Intracellular iron content significantly increased at 2, 4 and 24 hours in cells incubated with SPIONs (p