ANTIPROLIFERATIVE ACTIVITY OF HOP EXTRACTS IN TUMOR CELLS.

BATTISTA, ARIADNA; IBAÑEZ, IRENE; ARRUNÁTEGUI, LORENA; CERDÁN, MARCELO; POLICASTRO, LUCÍA; MOLINARI, BEATRIZ; DURÁN, HEBE

Pinamar

Congreso; X Congress of the Panamerican Association for Biochemistry and Molecular Biology (PABMB), XLI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology (SAIB); 2005

Panamerican Association for Biochemistry and Molecular Biology (PABMB) y Argentine Society for Biochemistry and Molecular Biology (SAIB)

Previous reports demonstrated multiple biological effects of humulones or alpha-acids from hop. The aim of this study was to evaluate the antiproliferative and antioxidant activity of hop extracts in human tumor cell lines (MCF-7, T47D, PAJU). Hop extracts used herein are pure, standardized solutions of iso-alpha-acids: hexahop gold (HH), tetrahop gold (TH) and isohop (IH) (Haas Hop Products, Inc.). Cell cultures were treated with the different extracts (0-100 ug/ml) for 24 h and dose-response curves were obtained. Proliferative activity was evaluated by MTT assay, apoptosis by staining with Hoescht33258 and antioxidant capacity by determining the oxidation of 2´,7´ dichlorofluorescin (DCFH). Results demonstrated a dose-dependent inhibition of cell growth in all the cell lines treated with HH (p