Characterization of the tolerogenic potential of T. cruzi-differentiated dendritic cells

PONCINI CV; BATALLA EI; ALBA SOTO C; GONZALEZ CAPPA STELLA M

Buenos Aires, Argentina

Congreso; 1er Congreso Franco-Argentino de Inmunología; 2010

SAI

Recently, we have demonstrated the ability of trypomastigotes (Tp) to modulate the differentiation stage and functionality of bone marrow-derived dendritic cells (DC) to a regulatory phenotype in vitro. Tp fail to activate DC and modulate LPS activation. DC display tolerogenic properties: they produce high levels of IL-10, less IL-12 and induce a poor alloresponse. In addition, we have demonstrated that both live and heat-killed Tp (Tphk) induce the regulatory phenotype in DC, characterized by an alternative activation state with enhanced ERK1/2 and STAT3 phosphorylation during LPS-stimulation. MEK inhibition led to a reduced production of IL-10 (p