CARDIAC KERNEL TRANSCRIPTION FACTOR NKX2-5 IS REGULATED BY A NOVEL SUPER-ENHANCER REGION IN PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES.

MARÍA AGUSTINA SCARAFIA; NICOLÁS POSTEGUILLO; SHEILA CASTAÑEDA; GUADALUPE AMÍN; MARÍA BELÉN PALMA; ALAN MIQUEAS MOBBS; JOAQUÍN SMUCLER; CAROLINA COLLI; LUCÍA MORO; ARIEL WAISMAN; ALEJANDRO LA GRECA; SANTIAGO MIRIUKA

Mar del Plata

Congreso; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); 2022

The decisions to commit towards a cardiac cell fate are taken earlyduring development. Defects in the cardiac program entail complicationsin cell specification and may lead to congenital heart diseases.Known as the cardiac kernel, TBX5, GATA4 and NKX2-5are key players of the gene regulatory network governing cardiacdifferentiation. However, the mechanisms involved in their regulationand the decision making process of cardiac commitment are stillnot clear. RNA-seq results of human induced pluripotent stem cells(hiPSC) differentiated to cardiomyocytes (CM) revealed 2.208 regulatedgenes (p-adj