CONICET | Buscador de Institutos y Recursos Humanos

The main goal of this research was to analyze in vitro compatibility of Nile tilapia (Oreochromisniloticus) digestive proteinases andenzymes recovered from species comprising fishery waste as Pleoticusmuelleri, Artemesialonginaris and Patagonotothenramsayi.It evaluated the use of exogenous enzymes as feeds supplements to increase digestion efficiency in Nile tilapia fingerlings and juveniles(3.5 ± 0.11 g and 11.6 ± 1.5 g, respectively). We successfully have obtained proteinases from fishery waste as source of exogenousenzymes, demonstrating thatproteinases can be easily extracted and employed as food additives to enhance the digestive process. P.muelleriand A. longinaris enzymes had more activity of acid and alkaline proteinases than P. ramsayi. SDS-PAGE gels demonstratedthat Nile tilapia digestive proteinases keep their activity when combined with each exogenous proteinase. Additionally, exogenousenzymes varied in their ability to enhance hydrolysis of different feed ingredients. In conclusion, P. muelleri by-products are thebest candidates to be employed as feed supplements for Nile tilapia juveniles. Enzymes from this by-product did not affect theactivity and integrity of fish digestive enzymes, improved the hydrolysis of several ingredients, and maintained its activity after beingexposed to high temperatures and acid pHs. Also we suggest supplementing first food of the day with exogenous enzymes to obtaintheir maximum activities. Fishery industry waste has a great biotechnological potential as source of proteinases. Our findings areapplicable to other places where O. niloticus is raised utilizing local fishery waste, and also to different cultured species.

enviar mensaje