Increased recruitment of granulocytes in the ovaries of dairy cows with cystic ovarian disease

STASSI, A.F.; ETCHEVERS, L.; ACOSTA, V.; VILLALBA L.; CAINELLI, S.; SALVETTI, N.R.; REY, F.; ORTEGA, H.H.

Mar del Plata

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

COD is among the most relevant cause of subfertility in dairy cattleand is the result of ovulation failure. Ovulation has been characterizedas an inflammatory process in which granulocytes are involved.Therefore, the aim of this study was to evaluate the distribution ofgranulocytes (CH138+) in ovaries of animals with spontaneousCOD and control (C) animals. Ovariectomy was performed in animalswith COD to obtain ovaries (n = 5), also control cows wereovariectomized in proestrus (n = 5). CH138+ cells were evaluatedthrough immunohistochemistry in total ovary (TO), in ovarian cortex(OC), medulla (OM), theca interna (TI) and externa (TE) of cystsand dominant follicles. In addition, CH138+ cells were evaluated byimmunocytochemistry performed on samples of follicular fluid from(FF) cysts and dominant follicles. The specificity of the antibody wascorroborated by western blot (WB). The data was analyzed usingANOVA with Duncan’s post-test and T-student test. WB analysisdetected a strong positive band of 17 KDa for CH138. Comparisonbetween groups showed that, the number of CH138+ cells washigher in TO, OM and TE of COD group than in C group (p