254. BONE REGENERATION WITH TERIPARATIDE-IMPREGNATED BOVINE BONE GRAFT FOR THE REPAIR CRITICAL-SIZED BONE DEFECTS IN RATS

PELLEGRINI, GRETEL G.; BONANNO, MARINA SOLEDAD; GONZÁLEZ CHAVES, MACARENA; ZENI CORONEL, ESTEFANÍA MAGALÍ; ORZUZA RICARDO; ZENI, SUSANA NOEMÍ; PAPAPANOU, PN

Mar del Plata

Congreso; Reunión Conjunta SAIC, SAB, AAFE, AACYTAL 2023; 2023

SAIC, SAB, AAFE, AACYTAL

Bone grafts are routinely used for bone defect repair in maxillofacial areas, and bovine bone (BB) is one of the most used bone grafting materials. Intermittently-administered parathyroid hormone 1-34 (PTH), or teriparatide (T), has anabolic effects on bone. We hypothesized that BB impregnated with T would enhance bone formation in the repairing of critical-sized bone defects (CSBD) in the tibiae of adult Wistar rats. To test this hypothesis, we evaluated the effect of T in impregnated BB particles on bone homeostasis; systemic bone remodeling; bone volume (BV) and microarchitecture; and new bone formation. The experimental protocol involved the following animal groups: Control CSBD without graft; BB: CSBD grafted with BB; BB & T: CSBD grafted with BB impregnated with T; BB & T + subcutaneous T (sc): CSBD grafted with BB impregnated T accompanied by injections of T for 30 days. Animals were sacrificed on day 45. Serum samples were obtained at multiple time-points to evaluate bone homeostasis and bone turnover markers. Bone microarchitecture by micro-CT and new bone formation by histomorphometry were evaluatedat the treated defects. Results: % change (D= at 45 vs. 0 days) of serum PTHi, amino-terminal propeptide of type I procollagen (P1NP) and carboxy-terminal telopeptide of type I collagen (CTX)were substantially and statistically significantly increased, while osteocalcin was decreased when compared to control (p