98. COMPARATIVE EFFECT OF GALACTOOLIGOSACCHARIDES (GOS) FROM TWO DIFFERENT DIETS MATRIX IN BONE HEATH OF GROWING RATS

SEIJO, MARIANA; BRYK, GABRIEL; ZENI CORONEL, ESTEFANÍA MAGALÍ; BONANNO, MARINA SOLEDAD; VÉNICA, CLAUDIA INÉS; PITA MARTÍN DE PORTELA, MARÍA LUZ; BERGAMINI, CARINA VIVIANA; WOLF, IRMA VERÓNICA; PEROTTI, MARINA CRISTINA; ZENI, SUSANA NOEMÍ

Mar del Plata

Congreso; Reunión Conjunta SAIC, SAB, AAFE, AACYTAL 2023; 2023

SAIC, SAB, AAFE, AACYTAL

In previous studies we demonstrated the effectiveness of GOS to increase calcium (Ca) absorption and bioavailability, and to improve bone retention. Objective: to compare the effect of galactooligosaccharides (GOS) from two different food matrix on bone formation and quality during normal growth in rats. Methods: Male weaning rats were divided in 3 groups and fed until 40 days of age: AIN-93G control diet (C), GOS mixed with fructooligosaccharides (FOS) in a proportion of GOS/FOS® 9:1 (PM) or GOS mixed with probiotic bacteria’s containing in an experimental yogurt diet (EY). Femur length (LF) (cm) and its Ca and phosphate (Pi) content; bone mineral density (BMD) and (BMC) of the total skeleton (tSk), lumbar spine (lSp) and proximal tibia (pT) by densitometry; bone volume percentage (BV%) and epiphyseal (GPC. Th) and hypertrophic (HpZ.Th)cartilages (μm) by histology; maximum fracture strength (N), and bone stiffness (N/mm) by biomechanical method. Shapiro-Wilk and Levene normality tests were applied, and ANOVA was performed to determine differences between groups using SPSS 19.0. p