SPEXIN PROMOTES WHITENING OF WHITE ADIPOSE TISSUE THROUGH MODULATION OF AUTOPHAGY

CASTRO, PATRICIA F.; LATINA CATALINA; CASTRO, AGUSTINA; GAMBARO, S.E.; GIOVAMBATTISTA, A.

Mar del Plata

Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2023

Spexin (SPX), a peptide adipokine, has been involved in many metabolicprocesses such as body weight regulation, food intake, energybalance and glucose and lipid metabolism. Recently, it was alsorelated to disrupt the thermogenic profile of brown and white adiposetissue (AT), but no information was found about its role during whiteadipose tissue whitening. For this purpose, C57BL/6J male micewere exposed 7 days at 4°C and then returned to room temperaturefor 8h or 24h where spx was administered (ip. 29 μg/kg; 8SPXw and24SPXw) or PBS (8CTRw and 24CTRw). Body weight and caloricintake were recorded daily. At the end of the experiment, plasmawas collected for Triglycerides (TG) measurement and EpididymalAT (EAT) and Inguinal AT (IAT) was dissected and weighted. IATwas also processed for qPCR gene expression (Ucp1; thermogenicmarker and Pink, Parkin, Atg12 and Atg7; autophagic markers)and for mitochondrial DNA (mitDNA) quantification. Two-way ANOVAwas used to determine variable (SPX and Time) and interaction(SPX x Time) effects. No differences in total caloric intake were observed but body weight change revealed that both SPX groups gainmore weight than CTR ones (P