Improvement in hyperactivation, capacitation and heterologous zona pellucida binding of cryopreserved equine spermatozoa after brief exposure to ionophore A23187

LAIZ QUIROGA L; NAVARRO MICAELA; OSYCKA-SALUT, C.; MUTTO A

Lima

Congreso; International Embryo technology Society; 2023

IETS

Spermatozoa must undergo three events: capacitation, hyperactivation and acrosome reactionto fertilize an oocyte. In different species, like murine and bovine, the calcium ionophoreA23187 has been used to study the in vitro acquisition of fertilizing ability in spermatozoa.A23187 increases events related to capacitation and hyperactive motility without inducing PKA-activation (pPKA) or protein tyrosine phosphorylation (pTyr). Therefore, it has been used toincrease the embryo production rate when combined with routine IVF protocols in thesespecies.