PROBIOTIC SUPPLEMENTATION IN RE-NUTRITIONAL DIETS IMPROVES CYTOKINE PROFILE IN THYMUS AND INTESTINE OF MALNOURISHED MICE

VELEZ, E.M.; FLORENCIA BALCELLS; CAZORLA, SILVIA INÉS; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

San Luis - Argentina

Congreso; LXXI ANNUAL MEETING OF THE ARGENTINEAN SOCIETY OF IMMUNOLOGY (SAI); 2023

Sociedad Argentina de Inmunologia

Malnutrition affects the intestinal barrier, specially the intestinal epithelial cells(IEC), and also influence the thymus (T) activity, leading to a dysfunction of theimmune response that can significantly increase morbidity and mortality frominfections.AIM: to evaluate the effect of nutritional recovery supplemented with differentprobiotic strain on the microenvironment of T and IEC through the production ofcytokines in malnourished mice.Methods: Balb/c mice were divided into groups according to the different dietsand supplementation: A) NC: Normal Control; B) DSN: Malnutrition; C) DSN+A:DSN+ conventional food; C) DSN+L: DSN+Milk; D) DSN+Lp: DSN+ LactobacillusParacasei 1518; E) DSN+M: DSN+ commercial probiotic MIX; F) DSN+A+L:DSN+ conventional food + Milk; G) DSN+A+Lp: DSN+ conventional food +Lactobacillus Paracasei 1518; and H) DSN+A+M: DSN+ conventional food +commercial probiotic MIX .Samples: T and IEC from mice of all experimental groups were taken and weperformed ex vivo cultures: IL-6, IFN-γ, TNF-α, IL-12 and IL-10 levels weremeasured by ELISA. Statistical analyzes were performed by GraphPad Prismsoftware using the ANOVA, with Tukey´s correction for multiple comparisons, p