An Electrochemical Platform for the Point-Of-Care Diagnosis of Infectious Diseases

MELLI, LUCIANO J.; CORTINA, MARIA E; REY SERANTES, DIEGO; UGALDE, JUAN E; CIOCCHINI, ANDRÉS E.; COMERCI, DIEGO J

Buenos Aires

Congreso; 15th International Meeting on Chemical Sensor (IMCS 2014); 2014

In this work, we present a point-of-care platform for the serologic diagnosis of infectious diseases. The principle of diagnosis can be regarded as an electrochemical enzyme-linked immunoassay. Different recombinant antigens were immobilized onto magnetic microparticles. After incubation with sera and horseradish peroxidase labeled conjugated antibodies, the magnetic particles were collected and placed in electrochemical cells where infected sera were amperometrically detected. All components of the platform were designed and developed during this project, including the electronic instrumentation and novel, disposable electrochemical cells, which allow eight simultaneous measurements. The platform was successfully tested for the diagnosis of foot-and-mouth disease, brucellosis, Chagas disease and infection by STEC O157.