Ultraviolet-B radiation modifies the methabolic profiles of terpenoids in in vitro Vitis vinifera L. cv. Malbec plants

GIL M; PONTIN M; BOTTINI R; PICCOLI P

Mendoza

Congreso; XXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2008

SBC

The ultraviolet-B radiation (UV-B) increases with altitude provoking damages at cellular levels. In this work, we analyzed the metabolic profiles of grape leaves subjected to an unique dose of UV-B and photosynthetic active radiation (PAR), versus control (PAR without UV-B).The plants were grown 40 days in modified MS-medium under PAR radiation (100 µmol m-2 s-1), after that submitted to a) 16 h PAR without UV-B (control); b) 16 h PAR + 16 h UV-B (7,2 µW cm-2). Then, the different metabolites were extracted from apical leaves with a gradient of polarity solvents. The extracts were analyzed by gas chromatography and mass spectrometry (GS-MS). In plants submitted to UV-B radiation the sesquiterpenes, Nerolidol, Bergamotene and Farnesol were identified, and those compounds were absent in the controls. Membranes sterols were by counterpart decreased. Our preliminary results suggest that the plant tissues expose to high intensities of UV-B synthesize de novo sesquiterpenes compounds, in detriment of sterols.