EFFECT OF SERUM FROM PATIENTS WITH ACUTE INTERMITTENT PORPHYRIA ON DELTA-AMINOLEVULINIC ACID SYNTHASE 1 PROTEIN EXPRESSION IN C3A HEPATOMA CELLS

MORA, SANDRA; OLIVERI, LEDA MARIA; PARERA, VICTORIA; BUZALEH, ANA MARIA; GEREZ ESTHER

Mar del Plata

Congreso; CONGRESO DE BIOCIENCIAS 2023; 2023

Sociedad Argentina de Investigación Clínica

ad Católica Argentina (UCA).Acute Intermittent Porphyria (AIP) is a hereditary disorder of hemebiosynthesis, characterized by a decreased activity of the enzymeporphobilinogen deaminase, associated with an induced expressionof delta-aminolevulinic acid synthetase 1 (ALA-S1), first enzyme andregulator of this pathway. In vitro investigations performed in ourlaboratory in C3A hepatoma cells, demonstrated that the addition ofhuman serum (HS) to culture medium caused a significant increasein ALA-S1 through Akt/mTOR/4EBP pathway. Based on these results,our aim was to extend the study using AIP patient’s serum(PHS) in order to elucidate the mechanisms underlying the deregulatio