EFFECT OF A DIET ENRICHED IN OLIVE OIL IN A KNOCKOUT MOUSE MODEL OF ACUTE INTERMITTENT PORPHYRIA

MARTINEZ MARIA DEL CARMEN; ZUCCOLI, JOHANNA; OLIVERI, LEDA MARIA; MORA, SANDRA; BUZALEH ANA MARIA; GEREZ ESTHER

Mar del Plata

Congreso; CONGRESO DE BIOCIENCIAS 2023; 2023

Sociedad Argentina de Investigación Clínica

Acute intermittent porphyria (AIP) is a hereditary disorder due to a decrease of porphobilinogen deaminase (PBG-D) activity; secondarily, 5-aminolevulic synthetase (ALA-S) is induced. Fasting is one of the triggering factors of acute attacks. To elucidate the mechanisms that lead to AIP onset and considering that a regulated diet can attenuate or prevent the attacks, the aim was to investigate if a diet rich in lipids affects heme biosynthesis. Male and female knockout mice deficient in PBG-D activity (Pbgd-/-) were used. For each sex, animals were divided in 3 groups: groups 1 and 2 received standard diet (Purina 3) for 12 weeks; group 3 was fed with standard diet supplemented with 15% commercial olive oil (20% of total lipids) during the same period; groups 2 and 3 were fasted 16 hours prior sacrifice. Serum glucose levels and lipid profile parameters (high- and low-density lipoproteins, total cholesterol and triglycerides) were measured. Initially the effect was evaluated through ALA-S activity/protein expression. In both sexes, oil did not alter the amount of food eaten, liver weight/body weight ratio or lipid profile compared to controls starved or not. Fasting caused 60% (p