Proteomic analysis of a native peanut phosphate solubilizing bacteria in response to P deficiency

LUDUEÑA LILIANA MERCEDES; CASTELLANI LUCAS; PEREZ GIMENEZ JULIETA; ANZUAY, MARIA SOLEDAD; TORRES TEJERIZO GONZALO; TAURIAN TANIA

Congreso; XXXI Reunión Latinoamericana de Rizobiología, VI Conferencia Latinoamericana de Microorganismos Promotores del Crecimiento Vegetal; 2023

Arachis hypogaea L. (peanut) is of a great economic importance in Argentina, being one of the top five exporting countries worldwide. In the peanut area of Argentina, low levels of P were detected, which has led to the search for sustainable strategies to provide this nutrient, such as the use of phosphate-solubilizing bacteria. These bacteria must be able to efficiently colonize the rhizospheric environment, thus, it is necessary to know the bacterial mechanisms involved in the response to P deficiency. The objective of the present study was to analyze the protein profile of the native peanut phosphate solubilizing model strain Serratia sp. S119 growing in P deficiency conditions. For this purpose, strain S119 was grown in Vincent medium containing tricalcium phosphate as the only source of P and the proteomic approach was done by shotgun-proteomics. Proteins were identified and quantified using DIA-NN software. It was possible to detect 2895 proteins corresponding to 60.4% of the total coding sequences annotated in the S119´s genome. Using the Perseus software and t-Student test, 61 proteins were detected that showed a fold change > 2 and p-value ≤ 0.05 in response to P deficiency, 43 overexpressed and 18 repressed. Preliminary analysis of the overexpressed proteins indicated that 9 of them are involved in iron metabolism and 8 in mobility and chemotaxis properties. This last characteristic is associated with previous results in which it was determined that S119 presents greater chemotaxis towards peanut plants in a P-deficient environment.