FUCOIDAN EXTRACT ISOLATED FROM UNDARIA PINNATIFIDA INHIBITS HSV 1-INDUCED NF-κB SIGNALING PATHWAYS ACTIVATION IN D407 CELLS AND PREVENTS THE BETA AMYLOID SYNTESIS.

GIULIANI MACARENA; UBOLDI CAMILA; LASSALLE, VERÓNICA L.; DELLATORRE, F; AYALA PEÑA V.B

Congreso; SAIC 2023; 2023

Currently, the use of antiviral drugs has been limited due to the generation of drug-resistant viral strains. Additionally, in certain age groups, their implementation is not recommended due to their high toxicity and limited effectiveness. In the present time, the trend in scientific and clinical research is towards the use of natural antiviral compounds as a more attractive option, as they are biodegradable and have minimal or no side effects. Fucoidans are sulphated polysaccharides obtained from brown seaweed. Studies have shown that certain fucoidans could reduce neurotoxic and inflammatory effects and have antiviral action. However, the role of fucoidans from Undaria pinnatifida on inflammation and amyloid beta peptide formation induced by viral infections in retinal pigment epithelial cells has not been explored yet.Objectives: To employ fucoidans derived from Undaria pinnatifida algae as potential antivirals and determine their role in inflammation and amyloid beta peptide formation in retinal pigment epithelial cells (D407). For this purpose, we used the HSV-1 virus, which is closely related to neurodegenerative pathologies. Results: the treatment with these fucoidans reduced viral titers in D407 cells, with an IC50: 0.8 µg/ml and a CC50: 736.9 µg/ml, resulting in an SI: 921.1. Additionally, we observed that treatment with a dose of 100 µg/ml reduced amyloid beta peptide synthesis by almost 100% at 24 hours by immunocytochemistry techniques, significantly inhibited the p65NFkB´s phosphorylation by western blot techniques, and reduced cell death by MTT assay. Additionally, a significant reduction of IL-6 was observed through real-time PCR techniques. These data together suggest that the fucoidan extract Isolated from Undaria pinnatifida Inhibits HSV 1-Induced NF-κB Signaling Pathways activation in D407 cells and prevents the beta amyloid formation.