PATERNAL PROGRAMMING OF ALTERATIONS IN THE PLACENTA OF FETUSES OF MALE DIABETIC RATS

PIRRONE, IRUNE; LEONARDI MARIA LAURA; JAWERBAUM, ALICIA; CAPOBIANCO, EVANGELINA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sociedad Argentina de Investigaciones Clínicas

The Paternal Origin of Health and Disease paradigm studies how paternal condition influences offspring health. Paternal Type 2 Diabetes programs metabolic alterations in the offspring. The placenta is involved in fetal programming, but it is unknown if it is affected by paternal diabetes. Peroxisome proliferator activated receptors (PPARs) are transcription factors needed for the appropriate development of the placenta. Aim: to study the paternal programming of alterations in the expression of PPARs in the placenta of fetuses in a model of paternal diabetic rats. Methods: Male control (C) and diabetic (D, streptozotocin-induced, 90 mg/kg) rats were mated with healthy females (n=8). Paternal sperm was collected from cauda epididymis to count spermatozoa and measure motility. On day 21 of gestation, pregnant rats were euthanized, and the placenta and the fetuses were sexed and weighed. In paternal and fetal plasma, glycemia, triglyceridemia (TG), and cholesterolemia (Ch) were measured by colorimetric assays. The placenta was stored at -80°C for the evaluation of PPARα, PPARγ and PGC1α (by Western blot). Results: Glycemia, TG and Ch was higher in D than in C males (P