Claudin-4 expression in placentas from a murine model infected with Salmonella enterica

BETANCOURT DIANA MARCELA; PUSTOVRH MARÍA CAROLINA; GARÓFALO AILIN; SARNACKI SEBASTIAN HERNÁN; GIACOMODONATO MÓNICA NANCY; NOTO LLANA MARIÁNGELES

Maceio

Congreso; X ENCUENTRO DE LA SOCIEDAD LATINOAMERICANA DE INTERACCIÓN MATERNO-FETAL Y PLACENTA (SLIMP) 2024; 2024

SOCIEDAD LATINOAMERICANA DE INTERACCIÓN MATERNO-FETAL Y PLACENTA (SLIMP)

Objective. To evaluate the expression and immunolocalization of claudin-4 (CLDN4) in placentas from a murine model infected with Salmonella enterica. Methods. 8-week-old female CF1 mice received a high dose of Salmonella enterica (SE) (2x108 CFU/mouse) intragastrically. After 20 days post-infection, females were mated with males of the same strain. At gestational day 18 (day 38 post-infection) the pregnant females were sacrificed and 2-3 placentas per female were removed. The expression and localization of claudin-4 (CLDN4) in control and infected placentas were determined by real-time PCR, immunohistochemical staining and immunofluorescence. All experimental protocols were approved by the Animal Ethics Committee, University of Buenos Aires. Results. In response to SE infection, the level of CLDN4 mRNA expression was significantly lower compared to placentas from the control group (p