INVESTIGADORES
ATORRASAGASTI FERNANDEZ Maria Catalina
congresos y reuniones científicas
Título:
Comparative transcriptional analysis of free fatty acid-treated hepatocyte primary culture uncovers the role of SPARC in lipotoxity
Autor/es:
LAMEROLI, LUCÍA; FIORE, ESTEBAN; BAYO, JUAN; CANTERO, MARIA JOSE; BUELONI, BARBARA; GARCIA, MARINA; ARGEMI, JOSEPMARIA; MAZZOLINI, GUILLERMO D.; ATORRASAGASTI, CATALINA
Reunión:
Congreso; LXVIII Reunión Científica anual de la Sociedad Argentina de Investigaciones Clínicas; 2023
Institución organizadora:
Sociedad Argentina de Investigaciones Clínicas
Resumen:
Hepatic fat accumulation is a hallmark of non-alcoholic fatty liver disease (NAFLD). Loss of hepatocytes capability to keep lipid homeostasis leads to dysregulation of lipid metabolism creating a lipotoxic environment which promotes NAFLD. The matricellular protein SPARC is involved in several pathophysiological processes such as inflammation, tissue remodeling and metabolism regulation. Our aim was to study the role of SPARC on hepatocytes response to saturated/unsaturated free fatty acids (FFA).Methods: Hepatocyte primary cultures from SPARC knockout (SPARC−/−) and wild type (SPARC+/+) mice were treated for 24h with 0.5 mM FFA (2:1 oleate and palmitic acid (PA) or only PA (0.2 mM) (n= 3/group). RNA was extracted and libraries were prepared to RNAseq analysis (Illumina). p-value
