Tomato Seed Transcriptome Changes Related To Phytochrome Control Of Germination.

G.A. AUGE; S. PERELMAN; R.A. SÁNCHEZ; J. BOTTO

Tampere

Congreso; XVI Congress of the Federation of European Societies of Plant Biology (FESPB 2008); 2008

Federation of European Societies of Plant Biology

Tomato seed germination can be inhibited by continuous irradiation with far-red light (FRc) and this inhibition can be relieved by a subsequent red light pulse (Rp). The information about the molecular mechanisms and signalling pathways involved in germination photocontrol is scarce. Using Potato microarrays, we carried out a global transcript analysis of wild type (cv. Money Maker) and phyA mutant seeds subjected to FRc inhibitory treatment with or without a subsequent Rp. A first analysis of global changes using microarray data showed that inhibition by FRc involves the induction of a large number of genes and the repression of a significantly smaller quantity. Correspondence Analysis showed an underlying pattern of expression dependent on the physiological treatment and incubation time. Canonical Discriminate Analysis identified different clusters of genes that could be associated with dormancy maintenance, inhibition and promotion of germination. A number of genes related to Gibberellins, ABA and other regulatory factors were differentially affected by the light treatments. We also found several genes like GIGANTEA, CSN6, ELIP and RBP that so far had been found associated to other physiological processes and might be involved in light-modulated germination as elements of phytochrome signalling.