“Modulation of C-type lectin receptors in dendritic cells by hydatid fluid from Echinococcus granulosus induces autophagy and polyfunctional T cell responses”

CHOP, MAIA; LEDO CAMILA; NICOLAO, MARÍA CELESTE; LOOS, JULIA; CUMINO, ANDREA C.; RODRIGUEZ RODRIGUES, CHRISTIAN

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias (SAIC-SAB-AAFE-AACyTAL); 2023

SAIC-SAB-AAFE-AACyTAL

Background: Cystic echinococcosis is caused by Echinococcus granulosus, which develops cysts that are filled with hydatid fluid (HF). C-type lectin receptors (CLRs) recognize carbohydrate structures leading to phagocytosis, autophagy and cytokine production. Autophagy plays a key role in protein presentation by dendritic cells, promoting T-cell activation. The aim of this work is to analyze if HF can be recognized by CLRs, polarizing the T-cell response in vitro. Methods: HF was collected from infected cattle. Autophagy induction was evaluated by FACS, qPCR and confocal microscopy in BMDCs. Expression of CD205 and Clec9a was evaluated by FACS. TFEB localization was analyzed by confocal microscopy. T-cell cytokine profiles were analyzed by qPCR. Results: HF-stimulated BMDCs significantly enhanced the number (**p