Development and characterization of metal and polymer nanoparticles for clinical diagnosis methods.

GABRIEL ALEJANDRO DE DIEGO; JORGELINA RODRIGUEZ GASTÓN; MARIEL ALEJANDRA FUSCO; FLORENCIA CONTI

Mar del Plata

Congreso; Reunión anual de Biociencia 2019; 2019

Sociedad Argentina de Investigación Clínica

The impact of advances of nanotechnology are particularly relevant in diagnostics, in which nanoparticle-based assays have been developed for specific detection of analytes of clinical interest. In particular, the use of magnetic nanoparticles attracts more attention because of their ability to be recovered and reused. Besides, the large surface area of the material allows adsorbing molecules of interest such as peptides, enzymes or antibodies needed to diagnose. The aim of this study is to develop a new method for the preparation of polymeric-nanoparticles with magnetic core to be used in a diagnostic device that detects target molecules in a biological sample. In order to achieve this goal, we synthesized nanoparticle magnetic covered with a polymeric matrix reactive to pH. Briefly, magnetic nanoparticles of cobalt ferrite (CoFe2O4) have been synthesized by the method of chemical coprecipitation with CoCl2 and FeCl3 solution. Later, a lax polymeric matrix was synthesized around the metal core using methacrylic acid solution. Then, the incorporation of a fluorescent dye was allowed in those nanoparticles. Finally, another denser polymeric matrix thin layer was added to the nanoparticles and antibodies of interest were adsorbed by them on their surface. We obtain spherical and monodisperse nanoparticles with diameter between 150 - 500 nm and a core of 65- 200 nm by DLS and TEM. FTIR was used to confirm links formation correspondent of each stage. The magnetic properties were investigated using a vibrating sample magnetometer. Here, the sample presented a superparamagnetic behavior, determined by the hysteresis cycle. Besides, we study the fluorescent dye release profile when the nanoparticles were exposed to different acidic pH. The polymeric matrix changes its conformation in 5.5 - 6.0 pH which allows to release the dye. This study shows a new synthesis of nanoparticles to use in a diagnostic test that presents a magnetic behavior and a polymer that can response a pH to release the dye.