ANTIOXIDANT, ANTIMICROBIAL AND CHEMICAL STUDIES OF ZUCCAGNIA PUNCTATA CAV. “JARILLA MACHO” DECOCTION AND ESSENTIAL OIL.

GÓMEZ JESSICA; ARIZA SAMPIETRO, M.B.; PESCHIUTTA, MARIA L.; SIMIRGIOTIS, MARIO J.; ALEJANDRO A. TAPIA; LIMA BEATRIZ

Congreso; XLI REUNION CIENTIFICA ANUAL DE LA SOCIEDAD DE BIOLOGIA DE CUYO; 2023

SOCIEDAD BIOLOGICA DE CUYO

This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential antioxidant and antibacterial properties of decoction (ZpDe) and Essential oil (ZpEO) from Zuccagnia punctata Cav. (Fabaceae, Caesalpinoideae). Metabolite profiling was obtained by an ultrahigh resolution liquid chromatography MS analysis (UHPLC-ESI-QTOF/OT-MS-MS) and GCMS. The antioxidant properties were screened by four methods: (2,2-diphenyl-1-picrylhydrazyl assay (DPPH), Trolox equivalent antioxidant activity assay (TEAC), ferric-reducing anti-oxidant power assay (FRAP), and lipid peroxidation in erythrocytes (ILP). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The ZpDe showed a strong DPPH scavenging activity (IC50 = 28.54 µg/mL); ILP of 87, 75% at 250 µg ZpDe/ml and moderated antimicrobial activity. The ZpDe showed a content of phenolics and flavonoids compounds of 241 mg GAE/g and 10 mg EQ/g respectively. On the other hand the ZpEO showed promising activity against a panel of yeasts Candida albicans and non albicans (ATCC and clinical isolated) with MIC values from 750 to 1500 µg/ml. Fifteen phenolics compounds were identified in ZpDe by ultrahigh resolution liquid chromatography (UHPLC–PDA– Q-TOF-MS) analysis, while twenty-nine compounds were identified in ZpEO means GC-MS analysis, updating the knowledge on the chemical profile of this species. The results support and standard out this medicinal plant mainly as a potential environmentally friendly and sustainable source of antioxidant and antimicrobial compounds.