Molecular diagnosis of toxigenic Fusarium species isolated from cereal grains of Argentina

SAMPIETRO D.A.; MARÍN, P.; IGLESIAS, J.; PRESELLO, D. A.; VATTUONE M.A.; CATALÁN, C. A. N.; GONZALEZ JAEN, M. T.

Tafi del Valle

Congreso; XXVII Annual scientific meeting of the Tucuman biology association; 2010

Tucuman biology association

Molecular biology techniques were assayed for species diagnosis and toxigenic potential of Fusarium isolated from cereals of Argentina. DNA was extracted from Fusarium isolates previously identified by morphological characters and / or mating type. PCRs based on intergenic spacer sequences (IGS) of rDNA and genes involved in fumonisins (FUM1) and trichothecenes (TRI13-TRI7) synthesis allowed us to detect and differentiate fumonisin producers (F. verticillioides and F. proliferatum). Non-fumonisin producers from Gibberella fujikuroi complex (F. subglutinans, F. andiyazi and F. thaspsinum) and trichothecene producers from the F. graminearum complex (F. graminearum sensu stricto, F. meridionale, F. boothii) were identified by the sequence of TEF1-alpha gene. F. meridionale and F. boothii were detected for the first time in northwest Argentina. PCRs based on TRI13 and TRI7 indicated that F. meridionale and F. boothii were nivalenol and deoxynivalenol producers, respectively. Molecular tools performed a reliable diagnosis and determination of toxigenic risk in the main species of Fusarium isolated from Argentina.