Lacticaseibacillus casei CRL75 MODULATES HUMORAL IMMUNE RESPONSE IN FOOD ALLERGY. PREVENTION OF THE DEVELOPMENT OF ASSOCIATED SYMPTOMS.

JONATHAN EMILIANO LAIÑO; MARÍA HORTENSIA ZELAYA; MARÍA PÍA TARANTO

San Luis

Congreso; LXXI REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2023

SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)

Food allergies constitute a public health problem of great importance as they can lead, in some cases, to anaphylactic shock. About 3% of adults and 3-8% of children suffer some type of this reactivity. In general, food allergy is not exclusive to one type of food, but rather many of them have cross-reactions with other foods and/or pollen. The beneficial effect of some lactic acid bacteria (LAB) on consumer health is widely reported, as well as the ability to release and/or produce specific compounds (nutraceuticals) with positive outcome on the human or animal host. Many exopolysaccharides (EPS) produced by beneficial LAB are powerful immunomodulatory molecules. Previously, it was demonstrated that Lacticaseibacillus (L.) casei CRL75 has a potent immunostimulatory effect and produces functional EPS. Objective: to evaluate the ability of L. casei CRL75 to prevent the development of symptoms associated to food allergies by regulating the humoral immune response and related cytokines. Materials and methods: The immunomodulatory capacity of L. casei CRL75 was evaluated in vivo by an OVA-induced intestinal allergy mouse model. Six week-old BALB/c mice were divided into 3 groups: a) Control (C): non-sensitized animals received a conventional balanced diet and water ad libitum; b) OVA: animals sensitized to OVA by bi-weekly intraperitoneal injection; c) L. casei CRL75 (CRL75): OVA-sensitized animals supplemented with L. casei CRL75 in drinking water for 7 days prior to OVA challenge. Two weeks after second injection, intestinal allergy was induced by feeding egg white diet for 7 days. Samples were obtained to evaluate: a) cytokines: IL-4, IL-10, TNF-α; b) OVA-specific antibodies (OD450nm): IgE, IgG, IgG1 and IgG2a; c) bacterial translocation to liver and spleen. Results: Supplementation with L. casei CRL75 induced the production of TNF-α, and IL-10, with a significant decrease in IL-4 levels (pg/ml, C: 71.43±18.1, OVA: 941±94.77; CRL75: 364.74±76.64, p