Dehydrin immunolocalization in quinoa (Chenopodium quinoa Willd.) embryos: distribution, sub-cellular localization and salt tolerance

JIMENA HEBE MARTINEZ; AXEL JOEL RIZZO; MARÍA BELÉN PALACIOS; HERNÁN PABLO BURRIEZA

Workshop; Imaging Techniques for Biotechnology and Biomedical Applications; 2016

Dehydrins (DHNs) are a group that belongs to the Late Embryogenesis Abundant protein (LEAs) family. DHNs are widelyspread across Plantae kingdom. Discovered in cotton seed embryos, were later found on numerous species and everyreproductive or vegetative tissue in plants. These proteins accumulate during late embryogenesis when it comes to orthodoxseeds, protecting them against the stress produced by desiccation during its maturation. In vegetative tissue, DHNs accumulateduring salt, drought and freeze stress. Every DHN presents one or more repeats of a 15 amino acid consensus sequence(EKKGIMDKIKEKLPG), called ?K-segment?. Previous reports show four DHNs (55, 50, 34 and 30 kDa) found in quinoaembryo belonging to Hualhuas genotype, being the lightest ones (34 and 30 kDa) capable of suffering phosphorylation.Present work studied DHNs accumulation in the embryos of six verifiable quinoa genotypes, in regards of salt tolerance. Inorder to do this, we used polyclonal antibodies against K-segment. Western-blots and immune essays were made. Formicroscopy essays, seed were hand cut and fixed in paraformaldehyde 4% solution, and included in LR-white resin. Materialwas cut in