CONICET | Buscador de Institutos y Recursos Humanos

During aging, T and B cells change their capacities to react against antigens. Few data is available whether DCs could participate on these changes. Here, we analyzed the phenotypic composition and function of DCs from aged (18-month old) Balb/c mice spleen. In spleen of aged mice, the proportion of CD11c+ cells was reduced, compared to their counterparts in young (3-month old) mice (in %, Exp1=1.7±0.1 vs 1.0±0.1; Exp2=1.3±0.1 vs 1.0±0.1), with an higher proportion of B220+ DCs over the whole population of DCs. In contrast, in mesenteric lymph nodes (MLN) from aged mice, DCs doubled the percentage observed in MLN form young mice (in %, Exp1=0.9±0.1 vs 1.7±0.1; Exp2=0.8±0.1 vs 1.5±0.3). Purified spleen DCs from aged mice, incubated overnight with oligodeoxynucleotides containing CpG motifs or with LPS produced more IL-10 and less IL-12p40 than DCs from young mice. In naïve conditions, splenic DCs from aged mice expressed slightly lower levels of MHC-II molecules. After i.v. injection of E.coli LPS, DCs from aged mice up-regulated MHC and co-stimulatory molecules at a lower extent than DCs from young DCs. Finally, DCs from young and aged mice stimulated equally an allogeneic response when young CD11c- cells were used as effector cells. Altogether, splenic DCs from aged mice show some changes that could affect the innate and adaptative immune response in spleen.E.coli LPS, DCs from aged mice up-regulated MHC and co-stimulatory molecules at a lower extent than DCs from young DCs. Finally, DCs from young and aged mice stimulated equally an allogeneic response when young CD11c- cells were used as effector cells. Altogether, splenic DCs from aged mice show some changes that could affect the innate and adaptative immune response in spleen.

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