COMBINATION OF PRENATAL ANDROGENIZATION AND HIGH-FAT DIET ON SEXUAL DEVELOPMENT AND ADULT REPRODUCTIVE FUNCTION OF FEMALE MICE

TORRES PEDRO JAVIER; MALDONADO ROCIO; RAMIREZ NICOLAS DAVID; PEREZ PABLO ANIBAL; LUQUE EUGENIA MERCEDES; CANTARELLI VERONICA INES; PONZIO MARINA FLAVIA; GUTIERREZ OSCHMAN SILVINA; MARTINI ANA CAROLINA

Encuentro; LXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2023

Polycystic ovary syndrome (PCOS) is frequently associated with femalesubfertility. Injecting dihydrotestosterone (DHT) into pregnantdams leads to a PCOS-like phenotype in the female offspring. Thisstudy aims to evaluate whether coadministration of a high-fat diet(HFD) to the pups exacerbates the effects of prenatal androgenizationand modifies plasma and ovarian Ghrelin (Ghrl) levels/expression.Pups received either a control diet (CD) or a HFD (commercialpellet + 30% pork fat) from weaning. We evaluated the impact offour treatments (DHT-CD; C-CD; DHT-HFD; and C-HFD) on sexualdevelopment, adult reproductive function, and plasma/ovarian Ghrllevels/expression (measured by western blot). Statistics: ANOVA(two-way or repeated measures); n=2-8 litters/treatment. No significantdifferences were found in pups’ weight gain. DHT delayedor even inhibited vaginal opening, with this effect being exacerbatedby the HFD (% vaginal opening on postnatal day 39: DHT-HFDa=25.0, DHT-CDbc=64.3, C-HFDb=100, C-CDbd=100; p