EXPRESSION LEVELS OF THE POTENTIAL REGULATORS OF FMR1, MIR-92A-3P AND MIR-19B-3P, DURING FOLLICULOGENESIS IN THE RAT

MARINA LUZ INGRAVIDI; LILIANA DAIN; LAURA KAMENETZKY; IANINA FERDER

Congreso; Reunión Conjunta SAIC SAB AAFE AACYTAL; 2023

The FMR1 gene is located on the X chromosome and is implicated, among othergenetic disorders, in the Fragile X-Associated Primary Ovarian Insufficiency. Severalstudies have shown dysregulated microRNA (miRNA) expression in patients withFragile X-associated diseases and that some miRNAs can potentially regulate FMR1expression. In previous in silico studies from our lab, we found 2 miRNAs, miR-92a-3p and miR-19b-3p, that are highly expressed in the rat’s ovary and are goodcandidates to bind to Fmr1.In the present work, we aimed to validate the expression of these 2 miRNAs duringfolliculogenesis, using a rat model of follicular development.We performed real time quantitative PCRs (RT-qPCR) using isolated RNA from rat’sovaries that were enriched in 3 different types of follicles: preantral follicles(prepubertal rats), early antral follicles (DES-treated rats) and preovulatory follicles(PMSG-treated rats). For each follicular stage, ovaries from 10-30 rats wererandomly divided in at least 4 different pools. Expression of each miRNA wasnormalized to U6. Additionally, we measured Fmr1 expression in the same follicularstages. Statistical analysis was performed using fgStatistics.We found that the expression of both miRNAs were 3-fold higher (p-value