EXPRESSION LEVELS OF THE POTENTIAL REGULATORS OF FMR1, MIR-92A-3P AND MIR-19B-3P, DURING FOLLICULOGENESIS IN THE RAT

MARINA LUZ INGRAVIDI; LILIANA DAIN; LAURA KAMENETZKY; IANINA FERDER

Congreso; Reunión Conjunta SAIC SAB AAFE AACYTAL; 2023

The FMR1 gene is located on the X chromosome and is implicated, among other genetic disorders, in the Fragile X-Associated Primary Ovarian Insufficiency. Several studies have shown dysregulated microRNA (miRNA) expression in patients with Fragile X-associated diseases and that some miRNAs can potentially regulate FMR1 expression. In previous in silico studies from our lab, we found 2 miRNAs, miR-92a-3p and miR-19b-3p, that are highly expressed in the rat’s ovary and are good candidates to bind to Fmr1.In the present work, we aimed to validate the expression of these 2 miRNAs during folliculogenesis, using a rat model of follicular development.We performed real time quantitative PCRs (RT-qPCR) using isolated RNA from rat’s ovaries that were enriched in 3 different types of follicles: preantral follicles (prepubertal rats), early antral follicles (DES-treated rats) and preovulatory follicles (PMSG-treated rats). For each follicular stage, ovaries from 10-30 rats were randomly divided in at least 4 different pools. Expression of each miRNA was normalized to U6. Additionally, we measured Fmr1 expression in the same follicular stages. Statistical analysis was performed using fgStatistics.We found that the expression of both miRNAs were 3-fold higher (p-value