POTENTIAL ANTIOXIDANT ACTIVITY OF AMARYLLIDACEAE SPECIES FROM PERU.

OLIMPIA LLALLA-CORDOVA; JAVIER ORTIZ; GABRIELA E. FERESIN; LORENA LUNA

San Juan

Congreso; XLI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2023

Sociedad de Biologia de Cuyo

lants produce secondary metabolites as defense compounds to protect themselves against abiotic (e.g., UV irradiation and desiccation) and biotic factors (herbivory and phytopathogenic microbial infections), as attractants for pollinators and seed-dispersing animals, as well as agents in plant–plant competition and plant–microbe symbiosis. The Amaryllidoideae subfamily of the maryllidaceae belongs to the order Asparagales, and consists of 14 tribes and about 70 genera. This family is widely distributed in different regions of the neotropics and temperate areas around the world. In Peru, 15 genera and 68 species belonging to the Amaryllidoideae have been reported in different types of forest, ranging from wet montane to dry, as well as the sandy biomes of the Pacific coastal region, with the greatest diversity in the south. The aim of this work was to determine the potential antioxidant of five species from Peru, through quantification of total phenolic and flavonoid content. In autumn 2023, bulbs of Hieronymiella spp., Pyrolirion albicans from Moquegua (Peru), and E. coccinea, E. darwinii, E. kawidei from the localities Cusco and Apurimac (Peru), were collected. From the dried bulbs, the methanolic extracts (MeOHE) were extracted by maceration at room temperature during 48 h, and concentrated under reduced pressure, and the percentage of yield (w/w) in terms of dry starting material was calculated. The total phenolic content (TFC) was determined by the Folin-Ciocalteau technique using a calibration curve with gallic acid, and the total flavonoid (FC) content was estimated by the aluminium chloride method with quercetin as standard both according to Luna et al. (2013). The yield values were Hieronymiella spp 7.01%, P. albicans 0.52%, E. coccinea 11.16%, E. darwinii 7.05%, and E. kawidei 2.51%.In E. coccinea and E. darwinii, CFTs were 11.44 ± 1.08 and 5.62 ± 0.87 mg EAG/100 g dry bulb) respectively, and were significantly different from E. kawidei (2.69 ± 0.87). E. coccinea and P. albicans showed the highest content of FC (2.02 ± 0.02 mg and 1.75±0.71 mg EQ/100 g of dry bulb, respectively). This is the first report regarding other metabolites such as phenolics and flavonoids, showing these compounds importance as responsible of the potential antioxidant properties in Amaryllidaceae species.Acknowledgement: Llalla-Cordova O. and Ortiz J.E. are scholarship CONICET. PICT03883 ANPCYT, PIP2022-2024-0902 (CONICET)AREA: NUTRICION Y SALUD. N° 11