COMPARATIVE EFFECT OF GALACTOOLIGOSACCHARIDES (GOS) FROM TWO DIFFERENT DIETS MATRIX IN BONE HEATH OF GROWING RATS

SEIJO, M; BRYK G; ZENI CORONEL EM; BONANNO, MS; VENICA, C; PITA MARTIN DE PORTELA ML; BERGAMINI, C; WOLF, I; PEROTTI M; ZENI SN

MAR DEL PLATA

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); SOCIEDAD ARGENTINA DE BIOLOGÍA (SAB); ASOCIACIÓN ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (AAFE); ASOCIACIÓN ARGENTINA DE CIENCIA Y TECNOLOGÍA DE ANIMALES DE LABORATORIO (AACYTAL)

increase calcium (Ca) absorption and bioavailability, and to improvebone retention. Objective: to compare the effect of galactooligosaccharides (GOS) from two different food matrix on bone formationand quality during normal growth in rats. Methods: Male weaningrats were divided in 3 groups and fed until 40 days of age: AIN-93Gcontrol diet (C), GOS mixed with fructooligosaccharides (FOS) ina proportion of GOS/FOS® 9:1 (PM) or GOS mixed with probiotic bacteria’s containing in an experimental yogurt diet (EY). Femurlength (LF) (cm) and its Ca and phosphate (Pi) content; bone mineral density (BMD) and (BMC) of the total skeleton (tSk), lumbar spine(lSp) and proximal tibia (pT) by densitometry; bone volume percentage (BV%) and epiphyseal (GPC. Th) and hypertrophic (HpZ.Th)cartilages (µm) by histology; maximum fracture strength (N), andbone stiffness (N/mm) by biomechanical method. Shapiro-Wilk andLevene normality tests were applied, and ANOVA was performedto determine differences between groups using SPSS 19.0. p