CHANGES IN BONE RESORPTION AND FORMATION MARKERS BY AGE AND DIFFERENT CONDITION THAT AFFECT BONE HEALTH. EXPERIMENTAL STUDY

ZENI CORONEL E M; BONANNO, MS; SEIJO, M; ZENI SN

MAR DEL PLATA

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); SOCIEDAD ARGENTINA DE BIOLOGÍA (SAB); ASOCIACIÓN ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (AAFE); ASOCIACIÓN ARGENTINA DE CIENCIA Y TECNOLOGÍA DE ANIMALES DE LABORATORIO (AACYTAL)

Bone is constantly being remodeled by bone cells coordinatedaction. A delicate equilibrium between bone formation by osteoblasts and resorption by osteoclasts is needed to maintain mineral homeostasis and biomechanical integrity. Activity of these bonecells called bone remodeling is biochemically assessed by specificbone turnover markers of bone formation (osteocalcin or OCN) andbone resorption (CTX). Bone remodeling changes with age and isregulated by hormonal and nutritional factors. Objective: Evaluatebone remodeling in different life stages and conditions that impactbone metabolism: age, estrogenic status, dietary calcium (Ca) and/or vitamin D nutritional status (VD) in female Wistar rats. Animalswere divided (n=8 per group) based on their age and estrogen deficiency: newborn (NB); weaning (RD); puberty (R45); young adult(R90 SHAM), late adulthood (R105SHAM), and estrogen deficiency(R90OVX, R105OVX) that fed a commercial diet. Effect of VD and/or Ca insufficiency was evaluated by feeding semi-synthetic dietsformulated according to AIN’93-M modified in vitamin D (D): 100IU%or 0UI% (+D and -D, respectively) and/or calcium: 0.5% or 0.3% (0.5and 0.3): R105OVX+D0.5; R105OVX-D0.5; R105OVX+D0.3, andR105OVX-D0.3. CTX and OCN were assessed by ELISA. ANOVAwas used., p