METABOLIC EFFECTS OF T47D CELLS IN MAMMA- RY ADIPOSE TISSUE

ROSA, FRANCISCO DAMIÁN; AIELLO, I; LIRA, MARÍA CECILIA; CASTELLANOS IARA; PALMA, ALEJANDRA; BERNACCHIA JULIANA LOURDES; PALADINO NATALIA; COSTAS M. A; RUBIO M F

Congreso; LXVIII ANNUAL MEETING OF SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2023

In previous work we have observed that TNF is able to induce li-polysis in adipose tissue (AT) explants from mammary glands ofC57-BL/6J mice and that the breast cancer cell line T47D secreteshigh levels of TNF. The aim of this work was to analyze the meta-bolic changes in AT produced by factors secreted for T47D cells.Wild type (wt) or TNF receptor 1 knock out (KO) AT explants werestimulated for 5 days with T47D cell line conditioned media (CM).The number of adipocytes per unit area of paraffin-embedded histo-logical sections was measured to assess adipocyte size. The T47DCM induced a decreased in adipocyte size (1.8 ± 0.06 respect to wtbasal, p