COMBINED GLYPHOSATE AND CHLORPYRIFOS BASED PESTICIDES ALTER BOTH INNATE AND ADAPTIVE IMMUNE EFFECTOR FUNCTIONS

ADRIÁN FRIEDRICH, , ; MARÍA SOFÍA AMARILLA; MARIANA GANTOV; MARÍA CECILIA SANTILLI; ALDANA TROTTA; DANIELA GONZÁLEZ PIÑERO; MARÍA VICTORIA REGGE; MARÍA NATALIA RUBINZSTAIN; BELÉN LOZADA MONTANARI; CAROLINA INÉS DOMAICA; MERCEDES FUERTES; NORBERTO WALTER ZWIRNER

Congreso; Reunion anual de la SAI; 2023

Glyphosate- and Chlorpyrifos-based pesticides (GbP and CbP respectively) are widely used inagriculture and are often applied to the same fields. There is evidence suggesting that GbP aregenotoxic and that both GbP and CbP are associated with an increased frequency ofmalignancies observed in highly fumigated rural areas. However, their effect onimmunosurveillance has been poorly explored. We have previously shown that GbP and CbPindividually alter NK cell function by reducing IFN- production after cytokine stimulation, andthat the combination of GbP+CbP affects T cell proliferation and the Th1 differentiation profile.Here, we focused on the mechanisms involved in the effects exerted by GbP and CbP on bothinnate and adaptive immune compartments. First, NK cells isolated from healthy donors werestained with CFSE and exposed to Gb, CbP or GbP+CbP, after which they were co-cultured for5h with eFluor670 dye labelled K562. Cells were harvested and stained with Zombie AquaViability Dye and analyzed by flow cytometry. GbP but not CbP treatment showed a reductionin NK cytotoxicity compared to control cells (p