Effect of cannabidiol (CBD) on HLA-G expression in human choriocarcinoma (JEG-3) tumor cells

MARTINEZ, KEVIN; PALMA, MARIA BELEN; CAROSELLA EDGARDO; GARCIA MARCELA; RICCILLO, FERNANDO

Mar del Plata

Congreso; Reunión Conjunta SAIC SAB AAFE AACYTAL 2023; 2023

Human leukocyte antigen G (HLA-G) is a protein known for its key role in fetal-maternal immune tolerance. It is expressed preferentially in fetal tissues and a restricted manner in certain healthy adult tissues. Different tumors express this protein, which gives them the ability to evade or decrease the immune response.There is extensive evidence on the palliative effects of cannabinoids (antiemetic, analgesic, anti-inflammatory, appetite stimulation, etc.) in patients with different tumors undergoing usual cancer treatments such as radiotherapy and chemotherapy. However, its therapeutic potential in oncology is not restricted to these effects. Several studies have demonstrated its antiproliferative, proapoptotic, antiangiogenic, and antimetastatic effects in various tumors, both in vivo and in vitro.Objectives. To evaluate the effects of different concentrations of cannabidiol (CBD) on the expression of HLA-G in the JEG-3 tumor line using molecular techniques.Materials and Methods. JEG-3 cells were cultured for 0, 12, 24 and 36 hours in the presence of 1 and 5 µM CBD, in DMEM + 0.4% DMSO medium, with their respective controls. After each incubation, HLA-G expression levels were analyzed by RT-PCR.Results. Through the t-test analysis (p