INVESTIGADORES
BARBEITO Claudio Gustavo
congresos y reuniones científicas
Título:
CARBOHYDRATE EXPRESSION IN UTERINE HORNS OF BALB/C MICE INFECTED WITH DIFFERENT STRAINS OF Tritrichomonas fetus
Autor/es:
WOUDWYK MA, MONTEAVARO CE DOUMECQ ML, ECHEVARRÍA H, CATENA M1, GIMENO EJ, SOTO P, BARBEITO CG
Lugar:
Buenos AIres
Reunión:
Jornada; Decimo primera Jornada Multidisplianria de la Sociedad Argentina de Biología; 2009
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
The bovine tritricomonosis is a venereal disease caused by the protozoan
Tritrichomonas foetus that produces embryonic death and
abortion. The protozoan strains isolated from different herds in the
country differ in their pathogenicity in Balb/c mice. In the pathogenesis
of the disease, cell adhesion mediated by the protozoan
surface glycoconjugates and host cells is very important. In a previous
work, we reported that the labelling intensity of SBA and
PNA lectins increased in luminal and glandular epithelia of the
uterus of mice infected with T. fetus. The aim of this work was to
confirm whether these changes are dependent on the strain of the
inoculated protozoa. We used preputial smegma samples of bulls
from Berker, Laprida, San Cayetano, Bolivar and Tres Arroyos,
which were inoculated intravaginally in Balb/c mice. Five weeks
after inoculation mice were sacrificed. Samples of uterine horns
were processed for lectinhistochemistry and incubated with SBA
and PNA lectins. In animals infected with the isolation from Bolivar
the affinity for both lectins in luminal and glandular epithelia of
the endometrium clearly increased. There is a partial relationship
between the lectin binding pattern and the previous data obtained
on the pathogenicity of the different strains.T. fetus. The aim of this work was to
confirm whether these changes are dependent on the strain of the
inoculated protozoa. We used preputial smegma samples of bulls
from Berker, Laprida, San Cayetano, Bolivar and Tres Arroyos,
which were inoculated intravaginally in Balb/c mice. Five weeks
after inoculation mice were sacrificed. Samples of uterine horns
were processed for lectinhistochemistry and incubated with SBA
and PNA lectins. In animals infected with the isolation from Bolivar
the affinity for both lectins in luminal and glandular epithelia of
the endometrium clearly increased. There is a partial relationship
between the lectin binding pattern and the previous data obtained
on the pathogenicity of the different strains.
